GDNF Protein (AA 78-211)

N° du produit ABIN7274722

Cette protéine Recombinant GDNF est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour GDNF Protein (AA 78-211) (ABIN7274722)

Antigène: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 78-211

Fonction: Human GDNF Protein

Séquence: Ser78-Ile211

Attributs du produit: Recombinant Human GDNF Protein is expressed from E.coli without tagIt contains Ser78-Ile211.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 0.1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human GDNF, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.7ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Autre désignation: GDNF

Sujet: Glial cell line-derived neurotrophic factor (GDNF) is a 134 amino acid protein belonging in the GDNF family ligands (GFLs). GDNF was originally isolated from rat glial cell lines and identified as a neurotrophic factor with the ability to promote dopamine uptake within midbrain dopaminergic neurons.

Poids moléculaire: 15.06 kDa same as Tris-Bis PAGE result.

UniProt: P39905-1

Pathways: Signalisation RTK, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration