LIF Protein (AA 23-202)

N° du produit ABIN7275151

Cette protéine Recombinant LIF est exprimée dans HEK-293 Cells.

Aperçu rapide pour LIF Protein (AA 23-202) (ABIN7275151)

Antigène: LIF (Leukemia Inhibitory Factor (LIF))

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 23-202

Fonction: Human LIF Protein

Séquence: Ser23-Phe202

Attributs du produit: Recombinant Human LIF Protein is expressed from HEK293 without tag.It contains Ser23-Phe202.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 0.1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human LIF at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human LIF R, hFc Tag with the EC50 of 0.39μg/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: LIF (Leukemia Inhibitory Factor (LIF))

Autre désignation: LIF

Sujet: Leukemia inhibitory factor (LIF) has played a vital role in a series of reproductive events, including follicle growth, embryo growth and differentiation. However, it is unclear whether the level of LIF in embryo culture medium can be used as a marker for clinical pregnancy.

Poids moléculaire: 19.7 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Tris-Bis PAGE result.

UniProt: P15018-1

Pathways: Signalistation JAK/STAT, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding