Mesothelin Protein (MSLN) (AA 406-690) (His tag)

N° du produit ABIN7275280

Cette protéine Recombinant Mesothelin est exprimée dans HEK-293 Cells.

Aperçu rapide pour Mesothelin Protein (MSLN) (AA 406-690) (His tag) (ABIN7275280)

Antigène: Mesothelin (MSLN)

Type de proteíne: Recombinant

Origine: Cynomolgus

Source: HEK-293 Cells

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 406-690

Purification/Conjugué: Cette Mesothelin protéine est marqué à la His tag.

Fonction: Cynomolgus MSLN/Mesothelin Protein

Séquence: Asp406-Gly690

Attributs du produit: Recombinant Cynomolgus MSLN/Mesothelin Proten is expressed from HEK293 with His tag at the C-Terminus.It contains Asp406-Gly690.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus MSLN at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-MSLN Ab., hFc Tag with the EC50 of 14.6ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: Mesothelin (MSLN)

Autre désignation: MSLN

Sujet: Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31- kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.

Poids moléculaire: 33 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Tris-Bis PAGE result.

NCBI Accession: XP_005590874

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process