TACSTD2
(Tumor-Associated Calcium Signal Transducer 2 (TACSTD2))
Type de proteíne
Recombinant
Attributs du protein
AA 27-274
Origine
Humain
Source
HEK-293 Cells
Purification/Conjugué
Cette TACSTD2 protéine est marqué à la His-Avi Tag,Biotin.
Fonction
Biotinylated Human TROP-2/TACSTD2 Protein
Séquence
His27-Thr274
Attributs du produit
Recombinant Biotinylated Human TROP-2/TACSTD2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His27-Thr274.
Pureté
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Anti-TROP-2 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human TROP-2, His Tag with the EC50 of 24.1ng/ml determined by ELISA. See testing image for detail.
TACSTD2
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
ELISA
TACSTD2
Origine: Humain
Hôte: Human Cells
Recombinant
>95 % as determined by reducing SDS-PAGE.
Active
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Date de péremption
12 months
Antigène
TACSTD2
(Tumor-Associated Calcium Signal Transducer 2 (TACSTD2))
Trop-2,also known as epithelial glycoprotein-1 antigen (EGP-1),is a protein that in humans is encoded by the TACSTD2 gene.Mutations of this gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness.
Poids moléculaire
30.5 kDa. Due to glycosylation, the protein migrates to 46-55 kDa based on Tris-Bis PAGE result.