VEGF 165 (AA 27-191) Protéine

N° du produit ABIN7275824

Protéine Recombinant exprimée dans HEK-293 Cells.

Aperçu rapide pour VEGF 165 (AA 27-191) Protéine (ABIN7275824)

Antigène: VEGF 165

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 27-191

Fonction: Human VEGF165 Protein

Séquence: Ala27-Arg191

Attributs du produit: Recombinant Human VEGF165 Protein is expressed from HEK293 without tag.It contains Ala27-Arg191.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 0.05 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human VEGF165 at 1μg/ml (100μl/well) on the plate. Dose response curve for Human VEGFR2, mFc Tag with the EC50 of 74.2ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: VEGF 165

Autre désignation: VEGF165

Sujet: Human papillomavirus (HPV) infection is an established risk factor for cervical carcinogenesis. VEGF165 was significantly higher, whereas VEGFC and VEGFD were significantly lower in malignant cervical carcinoma tissues as compared to normal cervix tissues. Expression levels of VEGF121 and VEGFC were significantly associated with type of tumor growth while VEGF165 was significantly associated with lymph node metastasis.

Poids moléculaire: 19.2 kDa. Due to glycosylation, the protein migrates to 20-30 kDa based on Tris-Bis PAGE result.

UniProt: P15692-4