VEGF 165 (AA 27-191) protein (His-Avi Tag,Biotin)

N° du produit ABIN7275828

Détail du produit

Antigène: VEGF 165

Type de proteíne: Recombinant

Attributs du protein: AA 27-191

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: His-Avi Tag,Biotin

Fonction: Biotinylated Human VEGF165 Protein

Séquence: Ala27-Arg191

Attributs du produit: Recombinant Biotinylated Human VEGF165 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala27-Arg191.

Pureté: > 95 % as determined by Tris-Bis PAGE

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human VEGF165 at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: VEGF 165

Autre désignation: VEGF165

Sujet: Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.

Poids moléculaire: 22.2 kDa. Due to glycosylation, the protein migrates to 28-35 kDa under reduced (R) condition, 45-60 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.