Flagellin Protein (FliC) (AA 1-495, active-Mutant)

N° du produit ABIN7566155

Cette protéine Recombinant Flagellin est produite dans Escherichia coli (E. coli).

Aperçu rapide pour Flagellin Protein (FliC) (AA 1-495, active-Mutant) (ABIN7566155)

Antigène: Flagellin (FliC)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Salmonella typhimurium

Source: Escherichia coli (E. coli)

Pureté: >95 % (SDS-PAGE)

Détail du produit

Attributs du protein: AA 1-495, active-Mutant

Fonction: Flagellin (rec.) (untagged) (highly active)

Réactivité croisée: Humain, Souris

Attributs du produit: Native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495).

niveau d'endotoxine: <0.001EU/μg purified protein (LAL test, Lonza).

Biological Activity Comment: Activation of TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, beta-defensin 2 and matrilysin (MMP-7). Maturation of dendritic cells in in vitro and in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous and intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin (rec.) (untagged) (highly active) at a concentration of 5-50ng/ml and in vivo at a starting concentration of 0.1-1μg/mouse.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 1 mg/mL after reconstitution.

Concentration: 1 mg/mL

Buffer: Contains PBS.

Conseil sur la manipulation: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution.

Stock: 4 °C,-20 °C

Stockage commentaire:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C.

Détail du antigène

Antigène: Flagellin (FliC)

Autre désignation: Flagellin

Sujet:

FliC

Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4).

Poids moléculaire: ~54kDa (SDS-PAGE)

UniProt: P06179

Pathways: Inflammasome