GDNF Protein (Biotin)

N° du produit ABIN7582099

Protéine Recombinant GDNF exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour GDNF Protein (Biotin) (ABIN7582099)

Antigène: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), Cell Culture (CC), Flow Cytometry (FACS), Live Cell Imaging (LCI), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Détail du produit

Purification/Conjugué: Cette GDNF protéine est marqué à la Biotin.

Fonction: human GDNF-Biotin

Séquence: SPDKQMAVLP RRERNRQAAA ANPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCDAAETTYD KILKNLSRNR RLVSDKVGQA CCRPIAFDDD LSFLDDNLVY HILRKHSAKR CGCI-OH

Specificité: Toxin target: GFRA1 (GDNF Receptor alpha 1), RET Receptor Tyrosine Kinase

Attributs du produit: LC-Biotin. The extent of labeling is 1-2 Molecules of biotin per one recombinant human GDNF protein, linked via primary amines.

Biological Activity Comment: GDNF enhances survival and differentiation of dopaminergic neurons. It is also a potent survival factor for motor neurons1-3.

Information d'application

Indications d'application: Antigen Preadsorption Control: 1 nM - 0.1 μM

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. The product is readily soluble in high-quality pure water at micromolar to millimolar concentrations (typically 5 μM to 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x the final working concentration. Divide the stock solution into small, single-use aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles to maintain optimal biological activity.

Buffer: Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).

Conseil sur la manipulation: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid multiple freeze-thaw cycles to maintain optimal biological activity,Avoid vigorous vortexing. protect from moisture

Stock: -20 °C

Stockage commentaire: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C and protect from moisture.

Détail du antigène

Antigène: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Autre désignation: GDNF

Sujet: A biotinylated human GDNF for imaging and sensitive detection of GFRα1/RET receptors and the study of dopaminergic neuron survival

Poids moléculaire: 30.8 kDa (dimer)

Pathways: Signalisation RTK, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration