Mesothelin Protein (MSLN) (AA 302-359) (His tag)

N° du produit ABIN7669293

Protéine Recombinant Mesothelin exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour Mesothelin Protein (MSLN) (AA 302-359) (His tag) (ABIN7669293)

Antigène: Mesothelin (MSLN)

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Immunogen (Imm)

Pureté: > 95 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 302-359

Purification/Conjugué: Cette Mesothelin protéine est marqué à la His tag.

Fonction: Recombinant Human MSLN/Mesothelin Protein (His Tag)

Séquence: Cys302-Lys359

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 10 EU/mg of the protein as determined by the LAL method

Biological Activity Comment: Not validated for activity

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: Mesothelin (MSLN)

Autre désignation: MSLN,Mesothelin

Sujet: CAK1,CAK1 antigen,MPF,MSLN,Megakaryocyte potentiating factor,Pre-pro-megakaryocyte-potentiating factor,SMR,mesothelin,soluble MPF mesothelin related protein,Mesothelin, also known as CAK1 and ERC, is derived from a 70 kDa precursor that also includes Megakaryocyte Potentiating Factor (MPF). The 70 kDa precursor is expressed on the cell surface where it is cleaved at a dibasic proteolytic site to release the 32 kDa glycosylated MPF. MPF is a cytokine that potentiates IL-3 induced megakaryocyte colony formation. The term Mesothelin refers to the 40 kDa glycosylated protein which remains attached to the cell surface via a GPI linkage. Alternate splicing generates additional Mesothelin isoforms that have either an eight amino acid insertion following Ser408 or a substituted C terminal region with no GPI anchor. This recombinant human Mesothelin lacks the 8 aa insertion, and within aa 296-580 it shares 59 % sequence identity with mouse and rat Mesothelin. Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas. It is up regulated in mesotheliomas and a range of carcinomas and adenomas. Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression. It is coexpressed with the tumor antigen CA125/MUC16 on advanced ovarian adenocarcinomas and interacts with this molecule to support cell adhesion. A soluble form of Mesothelin is released from tumor cells into the serum or tissue effusions.

Poids moléculaire:

Calculated MW: 6.3 kDa

Observed MW: 10 kDa

ID gène: 10232

UniProt: Q13421

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process