MANF Protein (AA 25-182)

N° du produit ABIN7866035

Protéine Recombinant MANF exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour MANF Protein (AA 25-182) (ABIN7866035)

Antigène: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: >95 % , as determined by SDS-PAGE and HPLC

Détail du produit

Attributs du protein: AA 25-182

Fonction: Human MANF Protein Recombinant

Séquence: LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL

Attributs du produit:

Protein Size: Recombinant MANF migrates as an approximately 17 kDa protein under reducing conditions in SDS-PAGE.

Sequence Position: Optimized DNA sequence encoding Human MANF mature chain including a C-terminal 6xHis tag was expressed in E. Coli. Recombinant Human Mesencephalic astrocyte-derived neurotrophic factor encodes amino acid residues 25-182.

niveau d'endotoxine: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).

Biological Activity Comment: Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 for this effect is 15-25 μg/ml.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Buffer: MANF was Lyophilized from PBS, pH 7.2.

Stock: 4 °C,-20 °C

Stockage commentaire: The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this product can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Date de péremption: 24 months

Détail du antigène

Antigène: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Autre désignation: MANF

Poids moléculaire: 17 kDa