Leptin Protein (LEP)
Aperçu rapide pour Leptin Protein (LEP) (ABIN7937227)
Antigène
Voir toutes Leptin (LEP) ProtéinesType de proteíne
Activité biologique
Origine
Source
Pureté
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Fonction
- Recombinant Human Leptin
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Séquence
- VPIQKVQDDT KTLIKTIVTR INDISHTQSV SSKQKVTGLD FIPGLHPILT LSKMDQTLAV YQQILTSMPS RNVIQISNDL ENLRDLLHVL AFSKSCHLPW ASGLETLDSL GGVLEASGYS TEVVALSRLQ GSLQDMLWQL DLSPGC
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Stérilité
- 0.2 μm filtered
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niveau d'endotoxine
- Less than 1 EU/ugof rHuLeptin as determined by LAL method.
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Biological Activity Comment
- Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human Leptin R transfected BaF3 murine proB cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 x105 IU/mg.
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Leptin (LEP) (AA 22-167) (Active) protein (hFc Tag)
LEP Origine: Souris Hôte: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Leptin (LEP) (AA 22-167) protein (GST tag)LEP Origine: Humain Hôte: Wheat germ Recombinant WB, ELISA, AP, AA
Leptin (LEP) protein (His tag)LEP Origine: Poisson rouge Hôte: Levure Recombinant > 95 % as determined by reducing SDS-PAGE.
Leptin (LEP) proteinLEP Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant >95 % as determined by reducing SDS-PAGE.
Leptin (LEP) (AA 22-167) protein (His-IF2DI Tag)LEP Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Leptin (LEP) (AA 22-167) protein (His tag)LEP Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Leptin (LEP) (AA 22-167) protein (His tag)LEP Origine: Porc Hôte: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Leptin (LEP) (AA 428-635) protein (His-IF2DI Tag)LEP Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Leptin (LEP) (AA 22-167) protein (Fc Tag)LEP Origine: Humain Hôte: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Leptin (LEP) (AA 29-164), (partial) protein (GST tag)LEP Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant 90 % ELISA
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Commentaires
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Approximately 16.0 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- Lyophilized from a 0.2 um filtered concentrated solution in 50 mm PB, pH 3.5, with 0.02 % Tween-20.
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Date de péremption
- 12 months
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- Leptin (LEP)
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Autre désignation
- Leptin
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Poids moléculaire
- 16.0 kDa
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ID gène
- 3952
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UniProt
- P41159
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Pathways
- Signalistation JAK/STAT, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
Antigène
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