BDNF Protein (AA 129-247)

N° du produit ABIN7937438

Cette protéine Recombinant BDNF est produite dans Insect cells (Sf21).

Aperçu rapide pour BDNF Protein (AA 129-247) (ABIN7937438)

Antigène: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Insect cells (Sf21)

Pureté: > 97 % by SDS-PAGE analyses.

Détail du produit

Attributs du protein: AA 129-247

Fonction: Recombinant Human/Murine/Rat/Canine/Equine Brain-derived Neurotrophic Factor

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Less than 0.1 EU/ug of rHu/Mu/Rt/Ca/EqBDNF as determined by LAL method.

Biological Activity Comment: Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 0.2-2 ng/mL. The specific activity of rHu/Mu/Rt/Ca/EqBDNF is approximately 1.3 x 10³units/ug, which is calibrated against recombinant human BDNF WHO Standard. Measured by its binding ability in a functional ELISA. When Recombinant Human TrkB Fc Chimera is coated at 1 ug/mL, rHu/Mu/Rt/Ca/EqBDNF binds with an apparent Kd <1 nM.

Information d'application

Commentaires:

Apparent molecular mass of 13-14 kDa in SDS-PAGE under reducing conditions, a single glycosylated polypeptide protein consisting of 119 amino acids.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from 0.2 um filtered concentrated solution in 100 mM Sodium Citrate and 300 mM NaCl, pH 3.0.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Date de péremption: 12 months

Détail du antigène

Antigène: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Autre désignation: Brain-derived Neurotrophic Factor

Poids moléculaire: 14 kDa

UniProt: P23560

Pathways: Signalisation RTK, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity