IRAK4 Protein (AA 154-460)

N° du produit ABIN7939195

Cette protéine Recombinant IRAK4 est exprimée dans Baculovirus infected Insect Cells.

Aperçu rapide pour IRAK4 Protein (AA 154-460) (ABIN7939195)

Antigène: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Type de proteíne: Recombinant

Origine: Humain

Source: Baculovirus infected Insect Cells

Application: Functional Studies (Func), Activity Assay (AcA), Screening Assay (ScA)

Détail du produit

Attributs du protein: AA 154-460

Fonction: Recombinant IRAK4 (154-460) protein

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Recombinant IRAK4 (154-460) protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Stock: -80 °C

Stockage commentaire: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Détail du antigène

Antigène: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Autre désignation: IRAK4

Sujet:

Protein Background: IRAK4 (Interleukin-1 receptor-associated kinase 4), also known as IMD67, IPD1, IRAK-4, NY-REN-64 or REN64. is a serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. IRAK4 also is a Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.

Short Description: Recombinant IRAK4 (154-460) protein that includes amino acids 154-460 of human IRAK4 protein (accession number NP_057207.2) was expressed in a baculovirus expression system and contains an N-terminal His and FLAG tag. The molecular weight of the protein is 38.89 kDa.

Aliases: IPD1, REN64, IRAK-4, NY-REN-64

Pathways: Signalisation NF-kappaB, Signalisation TLR, Activation of Innate immune Response, Toll-Like Receptors Cascades