KIT Protein (AA 546-976)

N° du produit ABIN7939264

Cette protéine Recombinant KIT est exprimée dans Baculovirus infected Insect Cells.

Aperçu rapide pour KIT Protein (AA 546-976) (ABIN7939264)

Antigène: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Type de proteíne: Recombinant

Origine: Humain

Source: Baculovirus infected Insect Cells

Application: Screening Assay (ScA), Functional Studies (Func), Activity Assay (AcA)

Détail du produit

Attributs du protein: AA 546-976

Fonction: Recombinant KIT / CD117 (546-976) protein

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Recombinant KIT (546-976) protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Stock: -80 °C

Stockage commentaire: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Détail du antigène

Antigène: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Autre désignation: KIT & CD117

Sujet:

Protein Background: KIT (KIT Proto-Oncogene, Receptor Tyrosine Kinase) is a tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. KIT phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL and activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KIT promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Diseases associated with KIT include Gastrointestinal Stromal Tumor and Piebald Trait.

Short Description: Recombinant KIT (546-976) protein that includes amino acids 546-976 of human KIT protein (accession number NP_000213.1) was expressed in a baculovirus expression system, and contains an N-terminal FLAG tag. The molecular weight of the protein is 50 kDa.

Aliases: KIT Proto-Oncogene, Receptor Tyrosine Kinase, CD117

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity