MGMT Protéine

N° du produit ABIN7939402

Protéine Recombinant MGMT exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour MGMT Protéine (ABIN7939402)

Antigène: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Détail du produit

Fonction: Recombinant MGMT protein

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Recombinant MGMT protein is supplied in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, 10 % glycerol and 0.5 mM TCEP.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Stock: -80 °C

Stockage commentaire: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Détail du antigène

Antigène: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Autre désignation: MGMT

Sujet:

Protein Background: MGMT(Methylated-DNA--protein-cysteine methyltransferase) Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Short Description: Recombinant MGMT protein that includes full length of human MGMT protein (accession number NP_002403.3) was expressed was expressed in E. coli cells with an N-terminal GST tag. The molecular weight of the protein is 48.48 kDa.

Aliases: O-6-Methylguanine-DNA Methyltransferase

Pathways: Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus