LKB1 Protéine

N° du produit ABIN7939544

Protéine Recombinant LKB1 exprimée dans Baculovirus infected Insect Cells.

Aperçu rapide pour LKB1 Protéine (ABIN7939544)

Antigène: LKB1 (STK11) (serine/threonine Kinase 11 (STK11))

Type de proteíne: Recombinant

Origine: Humain

Source: Baculovirus infected Insect Cells

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Détail du produit

Fonction: Recombinant STK11 / LKB1 protein

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Recombinant STK11 / LKB1 protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Stock: -80 °C

Stockage commentaire: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Détail du antigène

Antigène: LKB1 (STK11) (serine/threonine Kinase 11 (STK11))

Autre désignation: STK11 & LKB1

Sujet:

Protein Background: STK11 (Serine/Threonine Kinase 11) is a tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members and plays a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. STK11 phosphorylates the T-loop of AMPK family proteins, thus promoting their activity. STK11 phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK and also phosphorylates non-AMPK family proteins such as STRADA, PTEN and possibly p53/TP53. STK11 acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2 and thereby regulates processes including: inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, and B-cell differentiation in the germinal center in response to DNA damage.

Short Description: Recombinant STK11 / LKB1 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_000446.1) with an N-terminal FLAG tag. The molecular weight of the protein is 49.6 kDa.

Aliases: Serine / threonine kinase 11

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, L'effet Warburg