UBE2I Protéine

N° du produit ABIN7939702

Protéine Recombinant UBE2I exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour UBE2I Protéine (ABIN7939702)

Antigène: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Détail du produit

Fonction: Recombinant UBE2I protein

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Recombinant UBE2I protein is supplied in 25 mM Tris 8.0, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Stock: -80 °C

Stockage commentaire: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Détail du antigène

Antigène: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Autre désignation: UBE2I

Sujet:

Protein Background: UBE2I (SUMO-conjugating enzyme UBC9) also known as C358B7.1, P18, UBC9. UBE2I is a member of the E2 ubiquitin-conjugating enzyme family which is required for post-replicative DNA damage repair and plays an important role in various cellular processes. UBE2I can accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. Mediates sumoylation of ERCC6 which is essential for its transcription-coupled nucleotide excision repair activity.

Short Description: Recombinant UBE2I protein that includes full length of human UBE2I protein (accession number NP_003336.1) was expressed in E. coli and contains an N-terminal His tag with a molecular weight of 20.17 kDa. The purity of the protein is ≥ 95 % by SDS-PAGE.

Aliases: Ubiquitin conjugating enzyme E2 I, SUMO conjugating enzyme UBC9, C358B7.1, P18, UBC9, ubiquitin carrier protein 9, SUMO protein ligase, P18

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway