IGF1 Protein (AA 1-115)

N° du produit ABIN934921

Protéine Recombinant IGF1 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour IGF1 Protein (AA 1-115) (ABIN934921)

Antigène: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: > 95 % pure

Détail du produit

Attributs du protein: AA 1-115

Séquence: MGPETLCGAE LVDALQFVCG DRGFYFNKPT GYGSSSRRAP QTGIVDECCF RSCDLRRLEM YCAPLKPAKS A

Attributs du produit: Purified recombinant Human IGF1 protein
Expression System: E.coli
Bioactivity: The ED50 for this effect is < 3.6 ng/mL. Measured in a cell proliferation assay using MCF7 cell.
Molecular weight on SDS-PAGE will appear higher.

Information d'application

Indications d'application: IGF1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Procédure de l'essai:

Cell line: MCF7 (human) Maintenance Condition: RPMI 1640 containing 10 % FBS Assay Medium: serum free RPMI 1640 Cell Density: 2 x 10,000 cells/well (96 well plate, final volume 100 µL) Serum Free Starvation: 24 h with RPMI1640 Incubation Time: 40 hr (after sample treatment) Concentration Range: 0.39 ng/mL - 29 ng/mL Detection Method: BrdU assay

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Supplied as a liquid in PBS, pH 7.4.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: RT/-20 °C

Stockage commentaire: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Détail du antigène

Antigène: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Autre désignation: IGF1

Sujet: The Insulin-like growth factor-1(IGF-1) is a mitogenic polypeptide growth factors that stimulates the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. IGF-1 is predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGF-1 belongs to the insulin gene family, which also contains insulin and relaxin. The IGF-1s are similar in structure and function to insulin, but have a much higher growth-promoting activity than insulin. This recombinant human IGF-I is a globular protein containing 70 amino acids, and 3 intra-molecular disulfide bonds. Recombinant IGF-1 was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Alternative Names: MGF protein, IGF 1 protein, IGF-IA protein, Insulin like growth factor 1 protein, Insulin like growth factor 1 (somatomedin C) protein, Insulin like growth factor IB protein, IGF1 protein, IGFI protein, IGF IB protein, IGF1, IGF-1, IGF 1, IBP1 protein, IGF-1 protein, Insulin-like growth factor-1 protein, IGF1 protein, Mechano growth factor protein, IGF-1 protein, , Somatomedin C protein, IGFIA protein, Insulin like growth factor IA protein, Insulin-like growth factor-1 IGF IA protein, IBP1 protein, Somatomedin-C protein

Poids moléculaire: 7.7 kDa (71 AA)

Pathways: Signalisation RTK, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation