VEGFA Protéine

N° du produit ABIN988286

Cette protéine VEGFA est produite dans Escherichia coli (E. coli).

Aperçu rapide pour VEGFA Protéine (ABIN988286)

Antigène: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Activité biologique: Active

Origine: Souris

Source: Escherichia coli (E. coli)

Pureté: > 96 % by SDS-PAGE and HPLC analyses.

Détail du produit

Séquence: MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKCDKPR

Attributs du produit: Measured by its ability to induce proliferation of human umbilical vein endothelial cells. The ED50 for this effect is typically 2 - 4 ng/mL,corresponding to a specific activity of > 2.5×105 units/mg.

niveau d'endotoxine: Level Less than 1EU/µg of rmVEGF120 as determined by LAL method

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stock: 4 °C

Détail du antigène

Antigène: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Autre désignation: Vascular Endothelial Growth Factor 120 (VEGF120)

Sujet: VEGF was initially purified from media conditioned by normal bovine pituitary folliculo-stellate cells and by a variety of transformed cell lines as a mitogen specific for vascular endothelial cells. It was subsequently found to be identical to an independently discovered vascular permeability factor (VPF), which was previously identified in media conditioned by tumor cell lines based on its ability to increase the permeability of capillary blood vessels. Three mouse cDNA clones, which arise through alternative splicing and which encode mature mouse monomeric VEGF having 120, 164, or 188, amino acids, respectively, have been identified. Two receptor tyrosine kinases (RTKs), Flt-1 and Flk-1 (the mouse homologue of human KDR), both members of the type III subclass of RTKs containing seven immunoglobulin-like repeats in their extracellular domains, have been shown to bind VEGF with high affinity. The roles of the homodimers of KDR, Flt, and the heterodimer ofKDR/Flt in VEGF signal transduction remain to be elucidated.In vivo, VEGF has been found to be a potent angiogenesis inducer. Synonym: Vascular Endothelial Growth Factor 120 (VEGF120), Mouse. Formulation: Lyophilized from a 0.2µm filtered solution in PBS, pH 7.4.

Poids moléculaire: Recombinant mouse VEGF120 is a 28.4 kDa disulfide-linked homodimeric protein consisting of two 121 amino acid polypeptide chains.

Pathways: Signalisation RTK, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling