Measured by its ability to induce proliferation of human umbilical vein endothelial cells. The ED50 for this effect is typically 2 - 4 ng/mL,corresponding to a specific activity of > 2.5×105 units/mg.
Pureté
> 96 % by SDS-PAGE and HPLC analyses.
niveau d'endotoxine
Level Less than 1EU/µg of rmVEGF120 as determined by LAL method
VEGFA
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
VEGFA
Origine: Souris
Hôte: Yeast (Pichia pastoris)
Recombinant
> 95 % as determined by SDS-PAGE
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stock
4 °C
Antigène
VEGFA
(Vascular Endothelial Growth Factor A (VEGFA))
VEGF was initially purified from media conditioned by normal bovine pituitary folliculo-stellate cells and by a variety of transformed cell lines as a mitogen specific for vascular endothelial cells. It was subsequently found to be identical to an independently discovered vascular permeability factor (VPF), which was previously identified in media conditioned by tumor cell lines based on its ability to increase the permeability of capillary blood vessels. Three mouse cDNA clones, which arise through alternative splicing and which encode mature mouse monomeric VEGF having 120, 164, or 188, amino acids, respectively, have been identified. Two receptor tyrosine kinases (RTKs), Flt-1 and Flk-1 (the mouse homologue of human KDR), both members of the type III subclass of RTKs containing seven immunoglobulin-like repeats in their extracellular domains, have been shown to bind VEGF with high affinity. The roles of the homodimers of KDR, Flt, and the heterodimer ofKDR/Flt in VEGF signal transduction remain to be elucidated.In vivo, VEGF has been found to be a potent angiogenesis inducer. Synonym: Vascular Endothelial Growth Factor 120 (VEGF120), Mouse. Formulation: Lyophilized from a 0.2µm filtered solution in PBS, pH 7.4.
Poids moléculaire
Recombinant mouse VEGF120 is a 28.4 kDa disulfide-linked homodimeric protein consisting of two 121 amino acid polypeptide chains.