Reticulon 1A anticorps
Reactivité: Humain, Hamster, Souris, Rat
IHC (fro), IHC (p), IHC, WB, FACS, ICC
Hôte: Souris
Monoclonal
MON160
unconjugated
(5 références)-
- Antigène
- Reticulon 1A
- Reactivité
- Humain, Hamster, Souris, Rat
- Hôte
- Souris
- Clonalité
- Monoclonal
- Application
- Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)
- Specificité
- Human, mouse, hamster and rat.
- Purification
- Purified
- Immunogène
- MON-160 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with a partially purified bacterially expressed Reticulon-1A (NSP-A) hybrid protein (beta-GAL-NSP-A 6-776 ).
- Clone
- MON160
- Isotype
- IgG1
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- Indications d'application
- MON-160 exclusively recognizes the 135 kD Reticulon-1A protein in immunoblots of NCI-H82 and other SCLC cell lines, and stains normal and pathological neural and neuroendocrine tissues. The epitope of MON-160 is located between amino acid residues 174-337 of Reticulon-1A. MON-160 is useful for immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissue, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:50 - 1:100 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:50 - 1:500 for immunoblotting applications.
- Restrictions
- For Research Use only
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- Stock
- 4 °C
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: "A comparison of NSP-reticulons with conventional neuroendocrine markers in immunophenotyping of lung cancers." dans: The Journal of pathology, Vol. 182, Issue 1, pp. 13-21, (1997) (PubMed).
: "NSP-encoded reticulons, neuroendocrine proteins of a novel gene family associated with membranes of the endoplasmic reticulum." dans: Journal of cell science, Vol. 107 ( Pt 9), pp. 2403-16, (1995) (PubMed).
: "NSP-encoded reticulons are neuroendocrine markers of a novel category in human lung cancer diagnosis." dans: Cancer research, Vol. 54, Issue 17, pp. 4769-76, (1994) (PubMed).
: "Molecular analysis of expression in rat brain of NSP-A, a novel neuroendocrine-specific protein of the endoplasmic reticulum." dans: Brain research. Molecular brain research, Vol. 23, Issue 1-2, pp. 81-92, (1994) (PubMed).
: "Cloning and expression of alternative transcripts of a novel neuroendocrine-specific gene and identification of its 135-kDa translational product." dans: The Journal of biological chemistry, Vol. 268, Issue 18, pp. 13439-47, (1993) (PubMed).
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: "A comparison of NSP-reticulons with conventional neuroendocrine markers in immunophenotyping of lung cancers." dans: The Journal of pathology, Vol. 182, Issue 1, pp. 13-21, (1997) (PubMed).
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- Antigène
- Reticulon 1A
- Autre désignation
- Reticulon-1A / NSP-A
- Synonymes
- anticorps rtn1, anticorps rtn5, anticorps fb64c01, anticorps zgc:73298, anticorps zgc:86778, anticorps im:7136397, anticorps wu:fb64c01, anticorps wu:fc07e07, anticorps wu:fq24d12, anticorps zgc:113566, anticorps reticulon 1a, anticorps rtn1a
- Sujet
- Recently, a novel gene family has been identified and characterized, designated the Reticulons because the proteins encoded by these genes are anchored to the membranes of the endoplasmic reticulum. Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. Reticulon-1A (NSP-A), Reticulon-1B (NSP-B), and Reticulon-1C (NSP-C). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon-1A is a protein with a molecular weight (MW) of about 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).
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