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CD45 anticorps (FITC)

Cité dans 1 publication. Cet anticorps anti-CD45 Monoclonal Souris conjugué à FITC (Clone LT40) (ABIN135537) détecte spécifiquement CD45 dans FACS. L’anticorps est réactif avec des échantillons de Poulet.
N° du produit ABIN135537
612,67 €
Plus frais de livraison 40,00 € et TVA
500 μg
Destination: France
Envoi sous 7 à 10 jours ouvrables

Aperçu rapide pour CD45 anticorps (FITC) (ABIN135537)

Antigène

Voir toutes CD45 (PTPRC) Anticorps
CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Reactivité

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Poulet

Hôte

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Souris

Clonalité

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Monoclonal

Conjugué

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Cet anticorp CD45 est conjugé à/à la FITC

Application

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Flow Cytometry (FACS)

Clone

LT40
  • Specificité

    Chicken CD45, Mr 190-215 kDa

    Attributs du produit

    Mouse Anti-Chicken CD45-FITC

    Immunogène

    Chicken bursal lymphocytes

    Isotype

    IgM
  • Indications d'application

    • Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IHC-WM - Reported in literature , ICC - Reported in literature , IP - Reported in literature , Sep - Reported in literature
    • Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, and SPRD conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

    Volume d'échantillon

    1 mL

    Restrictions

    For Research Use only
  • Concentration

    0.5 mg/mL

    Buffer

    0.5 mg in 1.0 mL of PBS/Sodium azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Protect conjugated products from light.
    Each reagent is stable for the period shown on the bottle label if stored as directed.

    Stock

    4 °C

    Stockage commentaire

    Store at 2-8°C
  • Dalgaard, Norup, Pedersen, Handberg, Juorgensen, Juul-Madsen: "Flow cytometric assessment of chicken T cell-mediated immune responses after Newcastle disease virus vaccination and challenge." dans: Vaccine, (2010) (PubMed).

  • Antigène

    CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

    Autre désignation

    CD45

    Sujet

    Chicken CD45 is a transmembrane glycoprotein expressed on all leukocytes. B lineage cells in the bursa of Fabricius express the 215 kDa isoform while T lineage cells bear the 190 kDa variant. These high molecular weight molecules have intrinsic phosphotyrosine phosphatase activity characteristic of mammalian CD45.1 Levels of CD45 expression detected by MAb LT40 in the avian thymus are heterogeneous with approximately 90 % of thymocytes expressing fourfold higher levels of surface CD45 (CD45hi) than do the remaining 10 % (CD45lo) of thymocytes. The CD45lo population contains exclusively thymocytes with the CD3-CD4-CD8lo phenotype characteristic of the immediate precursors to the CD3-CD4+CD8+ thymic population which are CD45hi. This shift from low to high levels of surface CD45 expression is concomitant with the transition from CD4-CD8lo to CD4+CD8+ and before the expression of CD3.

    Pathways

    TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling
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