CD45 anticorps
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- Antigène Voir toutes CD45 (PTPRC) Anticorps
- CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
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Reactivité
- Humain, Chien
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD45 est non-conjugé
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Application
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Staining Methods (StM)
- Specificité
- Recognizes the CD45 leukocyte common antigen (LCA) family which is comprised of at least four isoforms of membrane glycoproteins (220, 205, 190, 180 kDa) expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues. The intracellular portions of these molecules have protein phosphatase activity and are involved in regulation of transmembrane signals. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms. A positive result with this MAb is highly indicative of lymphoid or myeloid origin. Certain types of lymphoid neoplasms may lack CD45 (Hodgkin lymphoma, some T-cell lymphomas, and some leukemias) so its absence does not rule out a hematolymphoid tumor. This antibody is expressed almost exclusively by cells of hematopoietic lineage and is present in most benign and malignant lymphocytes as well as plasma cell precursors.
- Purification
- Purified by Protein A/G
- Immunogène
- Isolated neoplastic cells from T cell lymphoma (2B11), and human peripheral blood lymphocytes maintained in T cell growth factor (PD7/26)
- Clone
- 2B11-PD7-26
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product PTPRC Anticorps primaire
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- Indications d'application
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Positive Control: Jurkat, Ramos, U-698 or GA-10 cells. Tonsil and Spleen.
Known Application: Flow Cytometry (0.5-2 μg/million cells), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-80 °C
- Stockage commentaire
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Date de péremption
- 24 months
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Investigation of canine extracellular vesicles in diffuse large B-cell lymphomas." dans: PloS one, Vol. 17, Issue 9, pp. e0274261, (2022) (PubMed).
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Investigation of canine extracellular vesicles in diffuse large B-cell lymphomas." dans: PloS one, Vol. 17, Issue 9, pp. e0274261, (2022) (PubMed).
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- Antigène
- CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
- Autre désignation
- PTPRC (PTPRC Produits)
- Synonymes
- anticorps PTPRC, anticorps si:dkeyp-47c5.1, anticorps B220, anticorps CD45, anticorps CD45R, anticorps GP180, anticorps L-CA, anticorps LCA, anticorps LY5, anticorps T200, anticorps Cd45, anticorps Ly-5, anticorps Lyt-4, anticorps loc, anticorps Lca, anticorps RT7, anticorps cd45, anticorps protein tyrosine phosphatase, receptor type C, anticorps protein tyrosine phosphatase, receptor type, C, anticorps receptor-type tyrosine-protein phosphatase C, anticorps PTPRC, anticorps ptprc, anticorps LOC100074271, anticorps Ptprc
- Poids moléculaire
- 180-220kDa
- ID gène
- 5788
- UniProt
- P08575
- Pathways
- TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling
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