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HIF1A anticorps (AA 610-727)

Name: HIF1A anticorps (AA 610-727)
SKU: ABIN968275
Availability: InStock

Cet anticorps Souris Monoclonal détecte spécifiquement HIF1A dans WB et IF. Il présente une réactivité avec des échantillons de Humain. Il a été cité dans 6+ publications.

Western Blotting (WB) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 610-727) antibody (ABIN968275) (HIF1A anticorps (AA 610-727))

Western blot analysis of HIF-1alpha on lysate from HeLa stimulated with CoCl2, cobalt chloride. (HIF1A anticorps (AA 610-727))

Image no. 3 for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 610-727) antibody (ABIN968275) (HIF1A anticorps (AA 610-727))

3 images

(6 références)

N° du produit ABIN968275

297,28 €

Plus frais de livraison 40,00 € et TVA

150 μg

Destination: France

Envoi sous 12 à 15 jours ouvrables

Aperçu rapide pour HIF1A anticorps (AA 610-727) (ABIN968275)

Antigène

Voir toutes HIF1A Anticorps

HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Reactivité

Humain

Hôte

Souris

Clonalité

Monoclonal

Conjugué

Cet anticorp HIF1A est non-conjugé

Application

Western Blotting (WB), Immunofluorescence (IF)

Clone

54-HIF

Discover our top product HIF1A Anticorps primaire

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AA 610-727

Attributs du produit

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène

Human HIF-1alpha aa. 610-727

Isotype

IgG1 kappa
anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 341-450) antibody
HIF1A Reactivité: Humain, Souris, Rat, Lapin WB, IP, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 703-732), (C-Term) antibody
HIF1A Reactivité: Humain, Souris, Rat WB, IHC Hôte: Lapin Polyclonal unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 575-826) antibody
HIF1A Reactivité: Humain WB, IHC, IP, ICC Hôte: Lapin Polyclonal unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 218-506) antibody
HIF1A Reactivité: Humain WB, IHC, IP, ICC Hôte: Lapin Polyclonal unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody
HIF1A Reactivité: Humain, Souris, Singe WB, IHC, ELISA, ICC Hôte: Souris Monoclonal 1A3 unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 519-547) antibody
HIF1A Reactivité: Humain, Souris WB, IF, FACS, IHC (p) Hôte: Lapin Polyclonal RB14548 unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 216-336) antibody
HIF1A Reactivité: Rat WB, IHC, IP, ICC Hôte: Lapin Polyclonal unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 329-530) antibody
HIF1A Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien WB, IHC, IP, ICC, IF Hôte: Souris Monoclonal ESEE122 unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 728-757) antibody
HIF1A Reactivité: Humain, Souris WB, IHC, ELISA, IF Hôte: Lapin Polyclonal unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 519-547) antibody
HIF1A Reactivité: Humain, Souris WB, IHC, ELISA, IF, FACS Hôte: Lapin Polyclonal unconjugated

Restrictions

For Research Use only
Format

Liquid

Concentration

250 μg/mL

Buffer

Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

-20 °C

Stockage commentaire

Store undiluted at -20°C.
Fallone, Britton, Nieto, Salles, Muller: "ATR controls cellular adaptation to hypoxia through positive regulation of hypoxia-inducible factor 1 (HIF-1) expression." dans: Oncogene, Vol. 32, Issue 37, pp. 4387-96, (2013) (PubMed).

Eckard, Dai, Wu, Jian, Yang, Chen, Costa, Frenkel, Huang: "Effects of cellular iron deficiency on the formation of vascular endothelial growth factor and angiogenesis. Iron deficiency and angiogenesis." dans: Cancer cell international, Vol. 10, pp. 28, (2010) (PubMed).

Arsham, Plas, Thompson, Simon: "Phosphatidylinositol 3-kinase/Akt signaling is neither required for hypoxic stabilization of HIF-1 alpha nor sufficient for HIF-1-dependent target gene transcription." dans: The Journal of biological chemistry, Vol. 277, Issue 17, pp. 15162-70, (2002) (PubMed).

Sánchez-Elsner, Botella, Velasco, Langa, Bernabéu: "Endoglin expression is regulated by transcriptional cooperation between the hypoxia and transforming growth factor-beta pathways." dans: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 43799-808, (2002) (PubMed).

Wiesener, Münchenhagen, Berger, Morgan, Roigas, Schwiertz, Jürgensen, Gruber, Maxwell, Löning, Frei, Maher, Gröne, Eckardt: "Constitutive activation of hypoxia-inducible genes related to overexpression of hypoxia-inducible factor-1alpha in clear cell renal carcinomas." dans: Cancer research, Vol. 61, Issue 13, pp. 5215-22, (2001) (PubMed).

Srinivas, Leshchinsky, Sang, King, Minchenko, Caro: "Oxygen sensing and HIF-1 activation does not require an active mitochondrial respiratory chain electron-transfer pathway." dans: The Journal of biological chemistry, Vol. 276, Issue 25, pp. 21995-8, (2001) (PubMed).
Antigène

HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Autre désignation

HIF-1alpha

Sujet

Molecular oxygen (O2) is essential for mammalian metabolic processes such as oxidative phosphorylation. Thus, survival depends upon instantaneous transcriptional modulation of genes that maintain O2 homeostasis. Transcriptional control of several of these genes is mediated by hypoxia-inducible factor 1 (HIF-1). HIF-1 is a heterodimer whose alpha and beta subunits are members of the PAS family of basic helix-loop-helix (bHLH) transcription factors. Common structural features of these proteins are an N-terminal bHLH DNA-binding domain and multiple PAS domains that confer dimerization ability and target gene specificity. Members diverge in their C-terminal regions. HIF-1beta is also known as the arylhydrocarbon nuclear translocator which is part of the functional dioxin receptor. However, HIF-1alpha functions exclusively to mediate responses to O2 deprivation. It contains C-terminal and internal transactivation domains. Although HIF-1 aproteinlevels increase during hypoxia, it is unstable in the presence of O2 due to an oxygen-dependent degradation domain (ODD) that targets it for ubiquitination. Thus, HIF-1alpha is essential for functional HIF-1 to mediate gene transcription in response to hypoxia. This antibody is routinely tested by western blot analysis.

Poids moléculaire

120 kDa

Pathways

Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, L'effet Warburg