Cet anticorps Lapin Polyclonal détecte spécifiquement NFKBIA dans WB, IHC et IF. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.
The antibody detects endogenous level of total IkB-a protein.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen. Specificity/Sensitivity IΰB-α (Ab-32/36) antibody detects endogenous levels of total IΰB-α protein.
Immunogène
Peptide sequence around AA 30-34, 34-38 (H-D-S-G-L- D-S -M-K) derived from Human IkB-a. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Western blotting: 1:500-1:1000 Immunohistochemistry: 1:50-1:100 Immunofluorescence: 1:100-1:200
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C/-20 °C
Stockage commentaire
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Antigène
NFKBIA
(Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))
Autre désignation
IkappaB-alpha
Sujet
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.