POLR2B anticorps (AA 805-854)
Aperçu rapide pour POLR2B anticorps (AA 805-854) (ABIN6736747)
Antigène
Voir toutes POLR2B AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
-
-
Épitope
- AA 805-854
-
Specificité
- Human POLR2B / RPB2
-
Homologie
- Percent identity by BLAST analysis: Human, Rat, Dog, Bovine, Xenopus (100%) Chicken, Zebrafish (92%).
-
Purification
- Immunoaffinity purified
-
Immunogène
-
Synthetic peptide located between aa805-854 of human POLR2B (P30876, NP_000929). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus, Xenopus (100%), Zebra finch, Chicken, Salmon, Stickleback, Zebrafish (92%).
Type of Immunogen: Synthetic peptide -
Isotype
- IgG
-
-
-
-
Indications d'application
-
Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. -
Commentaires
-
Target Species of Antibody: Human
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Reconstitution
- After adding water, will consist of PBS buffer with 2 % sucrose
-
Concentration
- Lot specific
-
Buffer
- Lyophilized from PBS with 2 % sucrose
-
Conseil sur la manipulation
- Avoid repeat freeze-thaw cycles.
-
Stock
- 4 °C,-20 °C
-
Stockage commentaire
-
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
-
-
- POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))
-
Autre désignation
- POLR2B / RPB2
-
Sujet
-
Name/Gene ID: POLR2B
Synonyms: POLR2B, HsRPB2, POL2RB, RNA polymerase II subunit B2, RNA polymerase II subunit 2, RPB2, HRPB140 -
ID gène
- 5431
-
NCBI Accession
- NP_000929
-
UniProt
- P30876
-
Pathways
- Regulatory RNA Pathways, Réparation de l'ADN
Antigène
-