POLR2B anticorps (Middle Region)

N° du produit ABIN2779656

Détail du produit anti-POLR2B anticorps

Antigène: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Épitope: Middle Region

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Lapin, Cobaye, Cheval, Poisson zèbre (Danio rerio)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLR2B est non-conjugé

Application: Western Blotting (WB)

Séquence: FFRSVFYRSY KEQESKKGFD QEEVFEKPTR ETCQGMRHAI YDKLDDDGLI

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Attributs du produit: This is a rabbit polyclonal antibody against POLR2B. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the middle region of human POLR2B

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 1174 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-POLR2B (ABIN2779656)

Lee, Skalnik: "Wdr82 is a C-terminal domain-binding protein that recruits the Setd1A Histone H3-Lys4 methyltransferase complex to transcription start sites of transcribed human genes." dans: Molecular and cellular biology, Vol. 28, Issue 2, pp. 609-18, (2008) (PubMed).

Détails sur POLR2B

Antigène: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Autre désignation: POLR2B (POLR2B Produits)

Synonymes: anticorps Pol2rb, anticorps Rpb140, anticorps Rpb2, anticorps POL2RB, anticorps RPB2, anticorps hRPB140, anticorps hsRPB2, anticorps RNA polymerase II subunit B, anticorps polymerase (RNA) II (DNA directed) polypeptide B, 140kDa, anticorps DNA-directed RNA polymerase II subunit RPB2, anticorps polymerase (RNA) II (DNA directed) polypeptide B, anticorps polymerase (RNA) II (DNA directed) polypeptide B, 140kDa L homeolog, anticorps POLR2B, anticorps polr2b, anticorps LOC100640211, anticorps Polr2b, anticorps polr2b.L, anticorps rpb-2

Sujet: POLR2B encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.
Alias Symbols: RPB2, POL2RB, hsRPB2, hRPB140
Protein Interaction Partner: ESR1, UBC, TCEB3, SRC, PSMB9, SUMO2, XRN2, WWOX, SUZ12, RNF2, NEDD4, ITCH, RABEP2, HECW2, FBXO6, CDK20, HDAC11, ILK, POLR2C, MED19, MED26, RECQL5, VCP, BARD1, MDC1, POLR3F, TUBB4B, TUBA4A, POLR2I, POLR2E, POLR2D, POLR2A, DCTN1, ATP6V1B2, MEN1, CDK7, CCNH,
Protein Size: 1174

Poids moléculaire: 134 kDa

ID gène: 5431

NCBI Accession: NM_000938, NP_000929

UniProt: P30876

Pathways: Regulatory RNA Pathways, Réparation de l'ADN