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GFP anticorps (Green Fluorescent Protein) (AA 183-191)

Détail du produit anti-GFP anticorps No. ABIN1742292, Fournisseur: Connectez-vous pour afficher
Antigène
Épitope
AA 183-191
44
28
19
18
6
4
3
3
2
2
1
1
1
1
1
1
Reactivité
Aequorea victoria
627
5
4
3
2
2
2
1
Hôte
Souris
310
168
87
43
16
9
8
2
Clonalité (Clone)
Monoclonal ()
Conjugué
Cet anticorp GFP est non-conjugé
33
32
28
15
13
8
8
7
6
6
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
517
332
186
141
134
124
69
61
43
26
14
13
10
9
8
6
6
4
4
3
3
3
3
3
2
2
2
1
1
1
1
1
Fournisseur
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Images

Immunogène Recombinant full length GFP, glutaraldehyde fixed. Epitope QQNTPIGDG (aa 183-191)
Clone 101G4
Isotype IgG2b
Specificité Specific for GFP of jelly-fish.
Purification purified IgG. Azide was added before lyophilization.
Autre désignation GFP
Domaine de recherche Tags/Labels
Indications d'application WB: 1 : 1000 up to 1 : 10 000 (AP staining)
IP: no (methanol fixated material only)
IHC: not tested yet
Commentaires

ABIN1742292 is recommended for IP and ICC.

Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS, 0.02% sodium azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Stock -20 °C
Stockage commentaire Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-GFP anticorps (Green Fluorescent Protein) (AA 183-191) (ABIN1742292) dilution: 1 : 1000, sample: recombinant protein
Produit citée dans: Koestler, Steffen, Nemethova, Winterhoff, Luo, Holleboom, Krupp, Jacob, Vinzenz, Schur, Schlüter, Gunning, Winkler, Schmeiser, Faix, Stradal, Small, Rottner: "Arp2/3 complex is essential for actin network treadmilling as well as for targeting of capping protein and cofilin." dans: Molecular biology of the cell, Vol. 24, Issue 18, pp. 2861-75, 2013 (PubMed).

Oelkers, Vinzenz, Nemethova, Jacob, Lai, Block, Szczodrak, Kerkhoff, Backert, Schlüter, Stradal, Small, Koestler, Rottner: "Microtubules as platforms for assaying actin polymerization in vivo." dans: PLoS ONE, Vol. 6, Issue 5, pp. e19931, 2011 (PubMed).