TGFA anticorps (C-Term)

N° du produit ABIN3026161

Détail du produit anti-TGFA anticorps

Antigène: TGFA (Transforming Growth Factor, alpha (TGFA))

Épitope: C-Term

Reactivité: Humain, Rat, Souris, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TGFA est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogène: A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF alpha was used as the immunogen for this antibody.

Clone: MF9

Isotype: IgG1 kappa

Information d'application

Indications d'application: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TGF alpha antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissues requires boiling sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.\. Western blot: 1-2 μg/mL, FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Stockage

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the TGF alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur TGFA

Antigène: TGFA (Transforming Growth Factor, alpha (TGFA))

Autre désignation: TGF alpha (TGFA Produits)

Synonymes: anticorps TFGA, anticorps LOC100009482, anticorps TGF alpha, anticorps TGFA, anticorps wa-1, anticorps wa1, anticorps RATTGFAA, anticorps TGFAA, anticorps NV15606, anticorps si:ch211-10e8.7, anticorps transforming growth factor alpha, anticorps transforming growth factor alpha L homeolog, anticorps transforming growth factor-alpha, anticorps transforming growth factor, alpha, anticorps TGFA, anticorps TGF-A, anticorps Tgfa, anticorps tgfa.L, anticorps LOC100121300, anticorps LOC100009150, anticorps tgfa

Sujet: TGF alpha is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. Staining with the antibody is completely blocked by the peptide used for raising this antibody.

ID gène: 7039

Pathways: Signalisation NF-kappaB, Signalisation RTK, EGFR Signaling Pathway