POLR3B anticorps (C-Term)

N° du produit ABIN311087

Détail du produit anti-POLR3B anticorps

Antigène: POLR3B (Polymerase (RNA) III (DNA Directed) Polypeptide B (POLR3B))

Épitope: C-Term

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Lapin, Poisson zèbre (Danio rerio), Cheval, Cobaye, Saccharomyces cerevisiae, Chévre

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLR3B est non-conjugé

Application: Western Blotting (WB)

Séquence: IEKVMISSNA EDAFLIKMLL RQTRRPEIGD KFSSRHGQKG VCGLIVPQED

Homologie: Cow: 100%, Dog: 100%, Goat: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 93%, Zebrafish: 100%

Attributs du produit: This is a rabbit polyclonal antibody against POLR3B. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the C terminal region of human POLR3B

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 1133 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-POLR3B (ABIN311087)

Yee, Gong, Huang, Lorent, Dolan, Maraia, Pack: "Mutation of RNA Pol III subunit rpc2/polr3b Leads to Deficiency of Subunit Rpc11 and disrupts zebrafish digestive development." dans: PLoS biology, Vol. 5, Issue 11, pp. e312, (2007) (PubMed).

Détails sur POLR3B

Antigène: POLR3B (Polymerase (RNA) III (DNA Directed) Polypeptide B (POLR3B))

Autre désignation: POLR3B (POLR3B Produits)

Synonymes: anticorps RGD1565311, anticorps C128, anticorps HLD8, anticorps RPC2, anticorps 2700078H01Rik, anticorps A330032P03Rik, anticorps C85372, anticorps RNA polymerase III subunit B, anticorps polymerase (RNA) III (DNA directed) polypeptide B, anticorps Polr3b, anticorps POLR3B

Sujet: POLR3B belongs to the RNA polymerase beta chain family. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR3B is the second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. It is proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft.
Alias Symbols: FLJ10388, RPC2, C128, HLD8
Protein Interaction Partner: SUMO2, UBC, POLR3H, TSR1, POLR1D, CRCP, POLR3A, POLR1C, POLR2H, POLR3D, RPAP2, RPAP1, GPN1, RAE1,
Protein Size: 1133

Poids moléculaire: 128 kDa

ID gène: 55703

NCBI Accession: NM_018082, NP_060552

UniProt: Q9NW08