MMP 9 anticorps

N° du produit ABIN337212

Cet anticorps anti-MMP 9 Polyclonal Lapin (ABIN337212) détecte spécifiquement MMP 9 dans WB, IHC, IP, IHC (p) et IHC (fro). L’anticorps est réactif avec des échantillons de Humain, Rat et Souris.

Aperçu rapide pour MMP 9 anticorps (ABIN337212)

Antigène: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MMP 9 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-MMP 9 anticorps

Specificité: Recognizes rat MMP-9 pro and active forms. Exhibits no cross-reactivity with other MMP family members.

Purification: Protein A purified

Immunogène: E. coli expressed active rat 92kD type IV collagenase (catalytic domain).

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-Fr (1:50), IHC-P (5 μg/mL), IP (1:250), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 1:500. Under fully reduced conditions, typically 98-95kD pro and 92-88kD active forms and breakdown products are detected. Under non-reduced conditions, additional bands may be detected due to SDS-stable TIMP and other inhibitor complexes. Positive control: HL660 cell lysate, U937 cell lysate, lung.

Commentaires:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles.

Détails sur MMP 9

Antigène: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Autre désignation: MMP9 / Gelatinase B

Sujet: Name/Gene ID: MMP9
Subfamily: Metallopeptidase M10A
Family: Protease

Synonyms: MMP9, 92kd gelatinase, 92 kd type iv collagenase, 92 kDa gelatinase, 92 kDa type IV collagenase, Matrix metalloproteinase-9, MMP-9, Type V collagenase, CLG4B, Gelatinase B, GELB, Macrophage gelatinase, MANDP2

ID gène: 4318

UniProt: P14780

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events