NFKBIA anticorps (pSer32, pSer36)

N° du produit ABIN362151

Cité dans 1 publication. Cet anticorps anti-NFKBIA Polyclonal Lapin (ABIN362151) détecte spécifiquement NFKBIA dans IHC et IF. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour NFKBIA anticorps (pSer32, pSer36) (ABIN362151)

Antigène: NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NFKBIA est non-conjugé

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-NFKBIA anticorps

Épitope: pSer32, pSer36

Specificité: The antibody detects endogenous level of IkB-a only when phosphorylated at serine 32/36.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: Peptide sequence around phosphorylation site of pSer32, 36 (H-D-S (p) -G-L-D-S (p) -M-K) derived from Human IkB-a. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Références pour anticorps anti-NFKBIA (ABIN362151)

Ren, Cui, Huo, Xue, Cui, Luo, Jiang, Yang: "Cordycepin suppresses TNF-?-induced NF-?B activation by reducing p65 transcriptional activity, inhibiting I?B? phosphorylation, and blocking IKK? ubiquitination." dans: International immunopharmacology, Vol. 14, Issue 4, pp. 698-703, (2012) (PubMed).

Détails sur NFKBIA

Antigène: NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))

Autre désignation: IkappaB-alpha

Sujet: Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.

Poids moléculaire: 39 kDa

NCBI Accession: NP_065390

UniProt: P25963

Pathways: Signalisation NF-kappaB, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Maintenance of Protein Location, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, BCR Signaling