SYK anticorps (AA 314-339)
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- Antigène Voir toutes SYK Anticorps
- SYK (Spleen tyrosine Kinase (SYK))
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Épitope
- AA 314-339
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp SYK est non-conjugé
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Application
- Western Blotting (WB), Flow Cytometry (FACS)
- Marque
- BD Pharmingen™
- Attributs du produit
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogène
- Human Syk peptide aa. 314-339
- Clone
- 4D10
- Isotype
- IgG2a
- Top Product
- Discover our top product SYK Anticorps primaire
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- Indications d'application
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Western Blot: EB1 control lysate [50 µg (1 µg/ml)] is provided as a positive control (store lysate at -20°C).
Additional application: The FITC-conjugated format of clone 4D10 is useful in immunofluorescent staining and flow cytometric analysis. - Commentaires
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Related Products: ABIN967389
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- SDS-PAGE buffer (62 mM Tris pH 6.8, 2 % SDS, 0.9 % b-mercaptoethanol, 0.003 % bromophenol blue, 5 % glycerol)
- Stock
- -20 °C
- Stockage commentaire
- Store undiluted at -20°C.
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Proximal protein tyrosine kinases in immunoreceptor signaling." dans: Current opinion in immunology, Vol. 13, Issue 3, pp. 299-306, (2001) (PubMed).
: "Tyrosine kinase SYK: essential functions for immunoreceptor signalling." dans: Immunology today, Vol. 21, Issue 3, pp. 148-54, (2000) (PubMed).
: "Pre-T cell receptor signals are responsible for the down-regulation of Syk protein tyrosine kinase expression." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 5, pp. 2610-20, (1999) (PubMed).
: "Critical role for the tyrosine kinase Syk in signalling through the high affinity IgE receptor of mast cells." dans: Oncogene, Vol. 13, Issue 12, pp. 2595-605, (1997) (PubMed).
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Proximal protein tyrosine kinases in immunoreceptor signaling." dans: Current opinion in immunology, Vol. 13, Issue 3, pp. 299-306, (2001) (PubMed).
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- Antigène
- SYK (Spleen tyrosine Kinase (SYK))
- Autre désignation
- Syk (SYK Produits)
- Synonymes
- anticorps p72-Syk, anticorps Sykb, anticorps p72syk, anticorps spleen associated tyrosine kinase, anticorps spleen tyrosine kinase, anticorps syk, anticorps SYK, anticorps Syk
- Sujet
- Syk is a member of the family of non-receptor type protein-tyrosine kinases and plays a crucial role in lymphocyte signaling and development. Syk is expressed in all haematopoietic cells and contributes to the signal transduction process by binding to a tyrosine-based activation motif (ITAM) of immune receptors, including Igalpha, TCRzeta, CD3epsilon, FcepsilonRIbeta, and FceRIgamma. Upon receptor activation, Syk binds to phosphorylated ITAMs via its two N-terminal SH2 domains thereby activating Syk and causing tyrosines in Syk to undergo auto-phosphorylation or phosphorylation. These phosphorylated sites can act as binding sites for other signaling molecules or help to regulate enzymatic activity. For example, in mast cells, Syk can activate downstream targets such as phospholipase Cgamma1 and VAV. Studies in Syk-/- mast cells identified defects in both the ERK-MAP and JNK-MAP kinase pathways. Syk migrates as 72 kDa in SDS/PAGE. (Predicted MW from SWISS-PROT is 72 kDa, Accession number P43405).
- Pathways
- Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Thromboxane A2 Receptor Signaling, BCR Signaling
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