Nitrite Determination Assay Kit
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- Méthode de détection
- Colorimetric
- Gamme de detection
- 1-100 μM
- Seuil minimal de détection
- 1 μM
- Application
- Biochemical Assay (BCA)
- Fonction
- The kit is designed for nitrite determination in biological samples.
- Type d'échantillon
- Plasma
- Specificité
- Specific for nitrite determination in plasma samples.
- Attributs du produit
- The overproduction of nitric oxide may lead to oxidative and nitrosative stress. It has been demonstrated that they enhance the development of a variety of diseases, as well as the ageing process1. Regarding nitrosative stress, high levels of iNOS have be
- Ingrédients
- Plate and the reagents neccesary to perform the assay.
- Matériel non inclus
- Pipettes, reaction tubes, plate reader.
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- Volume d'échantillon
- 50 μL
- Durée du test
- 1 h
- Plaque
- Uncoated
- Préparation des réactifs
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No reagent preparation is needed.
- Préparation de l'échantillon
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Plasma samples may be deproteinized before performing the assay.
- Procédure de l'essai
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Standard Preparation: Prepare the calibrate in 1 mL tubes following the Table 1 ( see in booklet) . Use ultrapure water as diluent. Performing the assay: 1. Add 50 μL of samples or standards in each well (96-well plate). 2.Add 50 μL of Reagent A in each well. Incubate for 10 minutes protected from light. 3.Add 50 μL of Reagent B in each well. Incubate for 10 minutes protected from light. 4.Read the absorbance at 540 nm within 30 minutes.
- Calcul des résultats
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Determine average absorbance value of each experimental sample. Determine its concentration by comparison to the Nitrite Standard reference curve (Figure 2 see images). Nitrite (μM) = (?A540nm- intercept)/ slope
- Restrictions
- For Research Use only
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- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
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