BLK Protein (AA 1-505) (His tag)

N° du produit ABIN667955

Cette protéine Recombinant BLK est produite dans Baculovirus infected Insect Cells.

Aperçu rapide pour BLK Protein (AA 1-505) (His tag) (ABIN667955)

Antigène: BLK (B Lymphoid Tyrosine Kinase (BLK))

Type de proteíne: Recombinant

Origine: Humain

Source: Baculovirus infected Insect Cells

Application: SDS-PAGE (SDS)

Pureté: > 80 %

Détail du produit

Attributs du protein: AA 1-505

Purification/Conjugué: Cette BLK protéine est marqué à la His tag.

Séquence: MGLVSSKKPD KEKPIKEKDK GQWSPLKVSA QDKDAPPLPP LVVFNHLTPP PPDEHLDEDK HFVVALYDYT AMNDRDLQML KGEKLQVLKG TGDWWLARSL VTGREGYVPS NFVARVESLE MERWFFRSQG RKEAERQLLA PINKAGSFLI RESETNKGAF SLSVKDVTTQ GELIKHYKIR CLDEGGYYIS PRITFPSLQA LVQHYSKKGD GLCQRLTLPC VRPAPQNPWA QDEWEIPRQS LRLVRKLGSG QFGEVWMGYY KNNMKVAIKT LKEGTMSPEA FLGEANVMKA LQHERLVRLY AVVTKEPIYI VTEYMARGCL LDFLKTDEGS RLSLPRLIDM SAQIAEGMAY IERMNSIHRD LRAANILVSE ALCCKIADFG LARIIDSEYT AQEGAKFPIK WTAPEAIHFG VFTIKADVWS FGVLLMEVVT YGRVPYPGMS NPEVIRNLER GYRMPRPDTC PPELYRGVIA ECWRSRPEER PTFEFLQSVL EDFYTATERQ YELQP

Attributs du produit: BLK, 1-505aa, Human, His tag, Hi-5

Purification: > 80% by SDS-PAGE

niveau d'endotoxine: < 1 EU per 1ug of protein (determined by LAL method)

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 7.5) containing 50 % glycerol, 0.25M NaCl, 5 mM DTT, 0.1 mM PMSF, 0.1 mM EDTA

Conseil sur la manipulation: Avoid repeated freezing and thawing cycles

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: BLK (B Lymphoid Tyrosine Kinase (BLK))

Autre désignation: Blk

Sujet: BLK, also known as B lymphoid kinase, is a 55 kDa tyrosine kinase with SH3, SH2, and catalytic domains that contain consensus sequences of the Src protein tyrosine kinase family. This protein is expressed specifically in the B cell lineage and plays a role in the signaltransduction pathway that is restricted to B lymphoid cells. Recombinant human BLK protein was expressed with N-terminal His-tag in High-Five cells using baculovirus expression system and purified by using conventional chromatography techniques.

Poids moléculaire: 59.8 kDa (525aa)

NCBI Accession: NP_001706

Pathways: Positive Regulation of Peptide Hormone Secretion, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling