anti-Phosphoinositide-Interacting Regulator of Transient Receptor Potential Channels (PIRT) Anticorps

Human Phosphoinositide-Interacting Regulator of Transient Receptor Potential Channels (PIRT) interaction partners

1. These results have uncovered species-specific TRPM8 modulation by PIRT. They provide evidence for a direct interaction between PIRT and the TRPM8 S1-S4 domain with a 1:1 binding stoichiometry, suggesting that a functional tetrameric TRPM8 channel has four PIRT-binding sites.

Mouse (Murine) Phosphoinositide-Interacting Regulator of Transient Receptor Potential Channels (PIRT) interaction partners

1. Using comparative electrophysiology experiments, the study identified species-dependent differences between the human and mouse TRPM8-PIRT complexes. It found that human PIRT attenuated human TPRM8 conductance, unlike mouse PIRT, which enhanced mouse TRPM8 conductance.

2. Results show that mechanical allodynia and thermal hyperalgesia were alleviated in Pirt-/- mice in chronic constriction injury (CCI) model. Taken together, Pirt together with transient receptor potential vanilloid channel 1 is involved in CCI-induced neuropathic pain.

3. Pirt regulates TRPM8 channel activity by increasing the single channel conductance.

4. These results demonstrate that Pirt is an endogenous regulator of P2X3 in bladder function.

5. Pirt plays an important role in mice uterine contraction-induced pain.

6. Pirt regulates the function of TRPM8 and its role in detecting cold.

7. Pirt contributes to both histaminergic and nonhistaminergic itch and, crucially, is involved in forms of itch that are both TRPV1-dependent and -independent.

8. Pirt is a key component of the TRPV1 complex and positively regulates TRPV1 activity.