Lapin anti-Boeuf (Vache) IgG (Heavy & Light Chain) Anticorps (Biotin) - Preadsorbed

N° du produit ABIN100901

Détail du produit

Antigène: IgG

Épitope: Heavy & Light Chain

Reactivité: Lapin, Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificité: IgG (H&L)

Attributs du produit: Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: Solid phase absorption

Immunogène:

Immunogen: Bovine IgG whole molecule

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Bovine IgG (H&L) has been assayed against 1.0 μg of Bovine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 μg of Bovine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Specific conditions for reactivity should be optimized by the end user.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Polyethylene Glycol (PEG-8000)

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Aliquot to Avoid repeated freezing and thawing.

Stock: RT,4 °C,-20 °C

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody

Sujet:

Synonyms: rabbit anti-Bovine IgG biotin Conjugated Antibody, rabbit anti-Bovine IgG Antibody biotin conjugation

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.