Chèvre anti-Cheval IgG (Heavy & Light Chain) Anticorps (TRITC) - Preadsorbed

Détails pour le produit réf. ABIN101409
Antigène
Épitope
Heavy & Light Chain
854
233
202
76
38
13
Reactivité
Cheval
246
226
173
156
135
80
80
57
54
53
48
41
40
38
25
16
14
13
8
7
7
3
2
1
Hôte
Chèvre
625
516
205
49
39
36
10
7
5
3
1
Clonalité
Polyclonal
Conjugué
TRITC
185
172
157
135
129
128
50
35
32
30
28
25
22
16
11
9
9
9
9
8
8
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
FLISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
Options
Immunogène

Immunogen: Horse IgG whole molecule

Isotype IgG
Specificité IgG (H&L)
Attributs du produit Concentration Definition: by UV absorbance at 280 nm
Purification Preadsorption: Solid phase absorption
Labeling Ratio 2.4
Antigène
Sujet

Synonyms: goat Anti-Horse IgG Biotin Conjugated Antibody, goat Anti-Horse IgG Antibody Biotin Conjugation

Background: Anti-Horse IgG Rhodamine Antibody generated in goat detects horse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Indications d'application

Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:500 - 1:2,500

IF Microscopy Dilution: 1:1,000 - 1:5,000

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 2.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Product is photosensitive and should be protected from light.
Stock RT,4 °C,-20 °C
Date de péremption 12 months