Myosin anticorps (pSer19, pSer20)

N° du produit ABIN104491

Détail du produit anti-Myosin anticorps

Antigène: Myosin

Épitope: pSer19, pSer20

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Myosin est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Attributs du produit: Concentration Definition: by UV absorbance at 280 nm

Immunogène: Human Myosin Light Chain phospho peptide corresponding to a region near the amino terminus of the human smooth/non-muscle form of myosin regulatory light chain conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Information d'application

Indications d'application: This phospho specific polyclonal antibody was tested by ELISA, immunohistochemistry and immunoblotting.   Immunoblotting was used to show reactivity with unstimulated and stimulated cardiac myocytes.  The antibody was also reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide.  Although not tested, this antibody is likely functional by immunoprecipitation.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.92 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Références pour anticorps anti-Myosin (ABIN104491)

Harbom, Rudisill, Michel, Litwa, Beenhakker, McConnell: "The effect of rho kinase inhibition on morphological and electrophysiological maturity in iPSC-derived neurons." dans: Cell and tissue research, Vol. 375, Issue 3, pp. 641-654, (2019) (PubMed).

Salomon, Gaston, Magescas, Duvauchelle, Canioni, Sengmanivong, Mayeux, Michaux, Campeotto, Lemale, Viala, Poirier, Minc, Schmitz, Brousse, Ladoux, Goulet, Delacour: "Contractile forces at tricellular contacts modulate epithelial organization and monolayer integrity." dans: Nature communications, Vol. 8, pp. 13998, (2018) (PubMed).

Panousopoulou, Hobbs, Mason, Green, Formstone: "Epiboly generates the epidermal basal monolayer and spreads the nascent mammalian skin to enclose the embryonic body." dans: Journal of cell science, Vol. 129, Issue 9, pp. 1915-27, (2017) (PubMed).

Logue, Cartagena-Rivera, Baird, Davidson, Chadwick, Waterman: "Erk regulation of actin capping and bundling by Eps8 promotes cortex tension and leader bleb-based migration." dans: eLife, Vol. 4, pp. e08314, (2016) (PubMed).

Newell-Litwa, Badoual, Asmussen, Patel, Whitmore, Horwitz: "ROCK1 and 2 differentially regulate actomyosin organization to drive cell and synaptic polarity." dans: The Journal of cell biology, Vol. 210, Issue 2, pp. 225-42, (2016) (PubMed).

Roland, Ricobaraza, Carrel, Jordan, Rico, Simon, Humbert-Claude, Ferrier, McFadden, Scheuring, Lenkei: "Cannabinoid-induced actomyosin contractility shapes neuronal morphology and growth." dans: eLife, Vol. 3, pp. e03159, (2015) (PubMed).

Solanes, Heuzé, Maurin, Bretou, Lautenschlaeger, Maiuri, Terriac, Thoulouze, Launay, Piel, Vargas, Lennon-Duménil: "Space exploration by dendritic cells requires maintenance of myosin II activity by IP3 receptor 1." dans: The EMBO journal, Vol. 34, Issue 6, pp. 798-810, (2015) (PubMed).

Fabbiano, Menacho-Márquez, Sevilla, Albarrán-Juárez, Zheng, Offermanns, Montero, Bustelo: "Genetic dissection of the vav2-rac1 signaling axis in vascular smooth muscle cells." dans: Molecular and cellular biology, Vol. 34, Issue 24, pp. 4404-19, (2015) (PubMed).

Edelson, Brautigan: "The Discodermia calyx toxin calyculin a enhances cyclin D1 phosphorylation and degradation, and arrests cell cycle progression in human breast cancer cells." dans: Toxins, Vol. 3, Issue 1, pp. 105-19, (2013) (PubMed).

Détails sur Myosin

Antigène: Myosin

Abstract: Myosin Produits

Synonymes: anticorps CG3201, anticorps DmMLC_C, anticorps Dmel\\CG3201, anticorps MyoII, anticorps mlc-c, anticorps nmELC, anticorps MYOSIN, anticorps myosin 1, anticorps DFNA4, anticorps DFNA4A, anticorps MHC16, anticorps MYH17, anticorps NMHC II-C, anticorps NMHC-II-C, anticorps PNMHH, anticorps myosin, anticorps MYH1, anticorps MYH2, anticorps MYH3, anticorps MYH4, anticorps MYH8, anticorps adult, anticorps embryonic, anticorps 2400004E04Rik, anticorps II-C, anticorps NHMCII, anticorps DROMHC, anticorps Dm nmII, anticorps DmnmII, anticorps Dronm-MII, anticorps E(br), anticorps MHC, anticorps Mhc-c, anticorps Mhc-c[1], anticorps Myo, anticorps Myo II, anticorps NMM, anticorps Zip, anticorps anon-WO0140519.37, anticorps l(2)02957, anticorps nmMHC, anticorps nmy-2, anticorps zpr, anticorps DmelCG15792, anticorps CG15792, anticorps Myosin light chain cytoplasmic, anticorps hypothetical protein, anticorps myosin 1, anticorps myosin heavy chain 9, anticorps myosin heavy chain 14, anticorps myosin, heavy chain 1B, skeletal muscle, anticorps myosin, heavy polypeptide 14, anticorps zipper, anticorps Mlc-c, anticorps T22C1.6, anticorps ATM1, anticorps MYH9, anticorps MYH14, anticorps Myh14, anticorps MYH1B, anticorps zip

Sujet: Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail.  The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons.  Activation of smooth and cardiac muscle primarily involves pathways that increase calcium levels and myosin phosphorylation, resulting in contraction.   Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain.  This antibody is specific for the phosphorylated form of myosin light chain.  The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells.  This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site.  Human and mouse have almost identical sequences.  In human the phosphorylation site is pS19, while in mouse the site maps to pS20.
Synonyms: Myosin regulatory light chain 12A Alternative name(s): Myosin regulatory light chain MRLC3 Myosin regulatory light chain 2, nonsarcomeric Myosin RLC MLC-2B

ID gène: 10627

UniProt: P19105