NR0B2 anticorps (Ligand Binding Domain)
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- Antigène Voir toutes NR0B2 Anticorps
- NR0B2 (Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2))
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Épitope
- Ligand Binding Domain
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Reactivité
- Humain, Souris, Lapin, Singe, Hamster
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp NR0B2 est non-conjugé
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Application
- Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marque
- IHC-plus™
- Specificité
- Human NR0B2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Homologie
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Hamster, Rabbit (100%) Rat, Bovine, Bat (94%) Elephant, Panda, Horse, Pig, Opossum (88%) Dog (81%).
- Purification
- Immunoaffinity purified
- Immunogène
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Synthetic 16 amino acid peptide from ligand-binding domain of human NR0B2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Hamster, Rabbit (100%), Rat, Bovine, Bat (94%), Elephant, Panda, Horse, Pig, Opossum (88%), Dog (81%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product NR0B2 Anticorps primaire
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- Indications d'application
- Approved: ICC, IF, IHC, IHC-P (4 μg/mL)
- Commentaires
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Target Species of Antibody: Human
- Procédure de l'essai
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- NR0B2 (Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2))
- Autre désignation
- NR0B2 / SHP (NR0B2 Produits)
- Synonymes
- anticorps SHP, anticorps SHP1, anticorps SHP-1, anticorps Shp1, anticorps Shp, anticorps NR0B2-A, anticorps Nr0b2, anticorps SHP-A, anticorps gb:bc058069, anticorps nuclear receptor subfamily 0 group B member 2, anticorps nuclear receptor subfamily 0, group B, member 2, anticorps nuclear receptor subfamily 0, group B, member 2a, anticorps NR0B2, anticorps Nr0b2, anticorps nr0b2a
- Sujet
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Name/Gene ID: NR0B2
Subfamily: NR0 Knirps-like
Family: NHR
Synonyms: NR0B2, Short heterodimer partner, SHP, Nuclear receptor SHP, Orphan nuclear receptor SHP, Small heterodimer partner, SHP1 - ID gène
- 8431
- Pathways
- Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway
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