NR5A1 anticorps (Internal Region)

N° du produit ABIN1049145

Détail du produit anti-NR5A1 anticorps

Antigène: NR5A1 (Nuclear Receptor Subfamily 5, Group A, Member 1 (NR5A1))

Épitope: Internal Region

Reactivité: Humain, Singe, Roussette (Chauve-souris)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NR5A1 est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human NR5A1. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except TRIM23 (47 % ).

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bat (100%) Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig (94%) Mouse, Rat, Hamster (88%) Turkey, Chicken, Platypus, Xenopus (82%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 17 amino acid peptide from internal region of human NR5A1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bat (100%), Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig (94%), Mouse, Rat, Hamster (88%), Turkey, Chicken, Platypus, Xenopus (82%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (2 - 18 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur NR5A1

Antigène: NR5A1 (Nuclear Receptor Subfamily 5, Group A, Member 1 (NR5A1))

Autre désignation: NR5A1 / SF1 (NR5A1 Produits)

Synonymes: anticorps AD4BP, anticorps ELP, anticorps FTZ1, anticorps FTZF1, anticorps POF7, anticorps SF-1, anticorps SF1, anticorps SPGF8, anticorps SRXY3, anticorps Ad4BP, anticorps ELP-3, anticorps Ftz-F1, anticorps Ftzf1, anticorps Ad4BP/SF-1, anticorps Ad4bp, anticorps Sf-1, anticorps NR5A1, anticorps sf-1, anticorps elp, anticorps sf1, anticorps ftz1, anticorps ad4bp, anticorps ftzf1, anticorps SF-1/Ad4BP, anticorps SI:zC167P9.2, anticorps ff1d, anticorps nr5a2l, anticorps nuclear receptor subfamily 5 group A member 1, anticorps nuclear receptor subfamily 5, group A, member 1, anticorps nuclear receptor subfamily 5 group A member 1 L homeolog, anticorps nuclear receptor subfamily 6 group A member 1, anticorps nuclear receptor subfamily 5, group A, member 1b, anticorps NR5A1, anticorps Nr5a1, anticorps nr5a1.L, anticorps nr5a1, anticorps NR6A1, anticorps nr5a1b

Sujet: Name/Gene ID: NR5A1
Subfamily: NR5 Fushi tarazu-like
Family: NHR

Synonyms: NR5A1, AD4BP, Adrenal 4 binding protein, Adrenal 4-binding protein, ELP, Steroidogenic factor-1, SRXY3, Steroidogenic factor 1, Nuclear receptor AdBP4, Nuclear receptor elp, POF7, SF-1, SPGF8, Steroid hormone receptor Ad4BP, STF-1, FTZ1, FTZF1, Fushi tarazu factor homolog 1

ID gène: 2516

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Maintenance of Protein Location