NR5A2 + LRH1 anticorps (C-Term)

N° du produit ABIN1049146

Détail du produit anti-NR5A2 + LRH1 anticorps

Antigène: NR5A2 + LRH1 (NR5A2) (Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2))

Épitope: C-Term

Reactivité: Humain, Boeuf (Vache), Mouton, Chien, Cheval, Porc, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NR5A2 + LRH1 est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human NR5A2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Sheep, Bovine, Dog, Elephant, Panda, Horse, Pig (100%) Opossum, Turkey, Chicken, Platypus, Xenopus, Stickleback (94%) Mouse, Rat, Trout, Pufferfish, Zebrafish (88%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 17 amino acid peptide from C-terminal to ligand-binding domain of human NR5A2. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Sheep, Bovine, Dog, Elephant, Panda, Horse, Pig (100%), Opossum, Turkey, Chicken, Platypus, Xenopus, Stickleback (94%), Mouse, Rat, Trout, Pufferfish, Zebrafish (88%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (7 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur NR5A2 + LRH1

Antigène: NR5A2 + LRH1 (NR5A2) (Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2))

Autre désignation: NR5A2 / LRH-1 (NR5A2 Produits)

Synonymes: anticorps xff1rashort, anticorps NR5A2, anticorps b1f, anticorps b1f2, anticorps cpf, anticorps ff1r, anticorps ftf, anticorps ftz-f1, anticorps ftz-f1beta, anticorps hb1f, anticorps hb1f-2, anticorps lrh-1, anticorps lrh1, anticorps xff1ra, anticorps B1F, anticorps B1F2, anticorps CPF, anticorps FTF, anticorps FTZ-F1, anticorps FTZ-F1beta, anticorps LRH-1, anticorps LRH1, anticorps hB1F-2, anticorps xFF1rB, anticorps AU020803, anticorps D1Ertd308e, anticorps Ftf, anticorps UF2-H3B, anticorps Lrh-1, anticorps nuclear receptor subfamily 5 group A member 2 S homeolog, anticorps nuclear receptor subfamily 5 group A member 2, anticorps nuclear receptor subfamily 5 group A member 2 L homeolog, anticorps nuclear receptor subfamily 5, group A, member 2, anticorps nr5a2.S, anticorps NR5A2, anticorps nr5a2, anticorps nr5a2.L, anticorps Nr5a2

Sujet: Name/Gene ID: NR5A2
Subfamily: NR5 Fushi tarazu-like
Family: NHR

Synonyms: NR5A2, B1F, B1F2, B1-binding factor, CPF, CYP7A promoter-binding factor, FTZ-F1beta, FTF, Liver receptor homolog 1, LRH1, LRH-1, HB1F, HB1F-2, Nuclear receptor phr-1, FTZ-F1, Liver receptor homolog-1, Nuclear receptor NR5A2, Receptor phr

ID gène: 2494

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway