PTGER3 anticorps (Cytoplasmic Domain)

N° du produit ABIN1049255

Détail du produit anti-PTGER3 anticorps

Antigène: PTGER3 (Prostaglandin E Receptor 3 (Subtype EP3) (PTGER3))

Épitope: Cytoplasmic Domain

Reactivité: Humain, Cheval, Singe, Boeuf (Vache), Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PTGER3 est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human PTGER3 / EP3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Bovine, Horse, Pig (100%) Gorilla, Rat, Elephant, Bat, Rabbit (94%) Mouse, Panda (88%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 16 amino acid peptide from 1st cytoplasmic domain of human PTGER3 / EP3. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Bovine, Horse, Pig (100%), Gorilla, Rat, Elephant, Bat, Rabbit (94%), Mouse, Panda (88%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (2 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur PTGER3

Antigène: PTGER3 (Prostaglandin E Receptor 3 (Subtype EP3) (PTGER3))

Autre désignation: PTGER3 / EP3 (PTGER3 Produits)

Synonymes: anticorps PTGER3, anticorps EP3, anticorps EP3-I, anticorps EP3-II, anticorps EP3-III, anticorps EP3-IV, anticorps EP3e, anticorps PGE2-R, anticorps PTGEREP3, anticorps Rep3, anticorps rEP3a, anticorps rEP3b, anticorps Pgerep3, anticorps Ptgerep3, anticorps prostaglandin E receptor 3, anticorps prostaglandin E receptor 3 L homeolog, anticorps prostaglandin E receptor 3 (subtype EP3), anticorps PTGER3, anticorps ptger3.L, anticorps Ptger3

Sujet: Name/Gene ID: PTGER3
Subfamily: Prostanoid
Family: GPCR

Synonyms: PTGER3, Ep3 prostanoid receptor, Ep3 receptor, EP3-I, EP3e, EP3-II, EP3-III, EP3-IV, PGE receptor, EP3 subtype, Prostaglandin receptor (PGE-2), Prostaglandin ep3 receptor, Prostanoid EP3 receptor, PGE receptor EP3 subtype, Pge2 receptor ep3, PGE2 receptor EP3 subtype, Prostaglandin E receptor 3, Prostaglandin e2 receptor ep3, EP3, Ep3 subtype pge2 receptor, PGE2-R, Prostaglandin E receptor EP3

ID gène: 5733