SLC5A6 anticorps (Internal Region)

N° du produit ABIN1049346

Détail du produit anti-SLC5A6 anticorps

Antigène: SLC5A6 (Solute Carrier Family 5 (Iodide Transporter), Member 6 (SLC5A6))

Épitope: Internal Region

Reactivité: Humain, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SLC5A6 est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human SLC5A6. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey (100%) Gibbon (94%) Marmoset (89%) Bat, Rabbit, Pig (83%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 18 amino acid peptide from internal region of human SLC5A6. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey (100%), Gibbon (94%), Marmoset (89%), Bat, Rabbit, Pig (83%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (1.25 - 2.5 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur SLC5A6

Antigène: SLC5A6 (Solute Carrier Family 5 (Iodide Transporter), Member 6 (SLC5A6))

Autre désignation: SLC5A6 / SMVT (SLC5A6 Produits)

Synonymes: anticorps SLC5A6, anticorps Slc5a6, anticorps E430023I20, anticorps SMVT, anticorps solute carrier family 5 member 8, anticorps solute carrier family 5 member 6, anticorps solute carrier family 5 (sodium-dependent vitamin transporter), member 6, anticorps SLC5A8, anticorps SLC5A6, anticorps Slc5a6

Sujet: Name/Gene ID: SLC5A6
Subfamily: Solute:sodium symporter
Family: Transporter

Synonyms: SLC5A6, SMVT

ID gène: 8884