NCKAP1 anticorps (Internal Region)

N° du produit ABIN1049510

L’anticorps Chèvre Polyclonal anti-NCKAP1 a été validé pour ELISA, IF et FACS. Il convient pour détecter NCKAP1 dans des échantillons de Humain.

Aperçu rapide pour NCKAP1 anticorps (Internal Region) (ABIN1049510)

Antigène: NCKAP1 (NCK Associated Protein 1 (NCKAP1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp NCKAP1 est non-conjugé

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-NCKAP1 anticorps

Épitope: Internal Region

Fonction: NCKAP1

Séquence: TPREYLTSHL EIR

Specificité: This antibody is expected to recognize both reported isoforms (NP_038464.1, NP_995314.1).

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-TPREYLTSHLEIR, from the of the protein sequence according to NP_038464.1, NP_995314.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Preliminary experiments gave an approx 100 kDa band in Human Cerebellum, Cerebral Cortex, Skin and Tonsil lysates after 0.5 μg/mL antibody staining, and was successfully blocked by incubation with the immunizing peptide. Please note the calcu

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur NCKAP1

Antigène: NCKAP1 (NCK Associated Protein 1 (NCKAP1))

Autre désignation: NCKAP1

Sujet: NCKAP1, NCK-associated protein 1, FLJ11291, HEM2, KIAA0587, MGC8981, NAP1, NAP125, NAP 1, OTTHUMP00000163460, OTTHUMP00000163461, membrane-associated protein HEM-2, nck-associated protein 1, p125Nap1

ID gène: 10787, 50884, 58823

NCBI Accession: NP_038464, NP_995314

Pathways: Signalisation RTK, Tube Formation, Asymmetric Protein Localization, Embryonic Body Morphogenesis