GFP anticorps (Alkaline Phosphatase (AP))

N° du produit ABIN1101745

Détail du produit anti-GFP anticorps (Alkaline Phosphatase (AP))

Antigène: GFP (Green Fluorescent Protein (GFP))

Reactivité: Aequorea victoria

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GFP est conjugé à/à la Alkaline Phosphatase (AP)

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificité: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Alkaline Phosphatase and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against Human, Mouse and Rat Serum Proteins.

Purification: Immunoaffinity purified

Stérilité: Sterile filtered

Immunogène: Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Type of Immunogen: Fusion protein

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA, IHC (1:200 - 1:1000), WB (1:500 - 1:2500)

Usage: Anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with a monoclonal anti-GFP antibody using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E. coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Aequorea victoria

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.05 M Tris chloride, 0.15 M sodium chloride, 1 mM magnesiuim chloride, 0.1 mM zinc chloride,  pH 8.0, 50 % glycerol, 1 % BSA, 0.1 % sodium azide, sterile filtered

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 4°C. Do not freeze.

Détails sur GFP

Antigène: GFP (Green Fluorescent Protein (GFP))

Autre désignation: GFP (GFP Produits)

Synonymes: anticorps green fluorescent protein, anticorps gfp

Classe de substances: Viral Protein

Sujet: Name/Gene ID: GFP

Synonyms: GFP

UniProt: P42212