HA-Tag anticorps (C-Term) (Biotin)

N° du produit ABIN1101939

Détail du produit anti-HA-Tag anticorps (Biotin)

Antigène: HA-Tag

Épitope: C-Term

Reactivité: Veuillez nous consulter SVP

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HA-Tag est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Séquence: YPYDVPDYA

Specificité: This affinity purified antibody is directed against the HA motif and is useful in determining its presence in various assays. This polyclonal anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag. To date this antibody has reacted with all HA tagged proteins so far tested. In western blotting of bacterial extracts the antibody does not cross-react with endogenous proteins. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Rabbit Serum.

Purification: Affinity purified

Immunogène: This antibody was purified from whole rabbit serum prepared by repeated immunizations with the 9-aa epitope tag peptide YPYDVPDYA (11 114-122) from hemagglutinin influenza conjugated to KLH using maleimide. A residue of cysteine was added to the carboxy terminal end to facilitate coupling.

Type of Immunogen: Purified protein

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA, IHC (1:1000 - 1:5000), WB (1:2000 - 1:10000)

Usage: Anti-HA is optimally suited for monitoring the expression of HA tagged fusion proteins. As such, anti-HA/HA can be used to identify fusion proteins containing the HA epitope. The antibody recognizes the HA epitope tag fused to the amino- terminus of targeted proteins as is expressed in many commonly used expression vectors. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and HA containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry, and other immunodetection techniques. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins. Biotin conjugated anti-EPITOPE tag antibodies are generally suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: deionized water

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 1 % BSA, 0.01 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: avoid freeze thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Détails sur HA-Tag

Antigène: HA-Tag

Autre désignation: Hemagglutinin / HA Tag (HA-Tag Produits)

Classe de substances: Tag

Sujet: Name/Gene ID: HA Tag

Synonyms: HA Tag