CDK6 anticorps

N° du produit ABIN112144

Cet anticorps Souris Monoclonal détecte spécifiquement CDK6 dans WB, IHC (p), IP et IHC (fro). Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour CDK6 anticorps (ABIN112144)

Antigène: CDK6 (Cyclin-Dependent Kinase 6 (CDK6))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CDK6 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: DCS-83

Détail du produit anti-CDK6 anticorps

Specificité: This antibody reacts specifically with Cdk6 kinase, which can substitute for Cdk4. Cdk6 is highly expressed in some lymphoid tumor and sarcoma cell lines (ML-1 and U-2-OS resp.). Positive staining is found in head and neck tumors.

Purification: Protein A affinity chromatography

Immunogène: Human recombinant full-length cdk6 polypeptide

Isotype: IgG1

Information d'application

Indications d'application: Immunohistochemistry on frozen sections and paraffin embedded tissue (after microwavetreatment): 1: 10 for 1 - 4 h at RT or overnight at 2-8C. Western Blot. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in 1 mL dist. water.

Buffer: PBS, pH 7.4, containing 0.09 % NaN3, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C up to one week or (in aliquots) at -20 °C for longer. Centrifuge vial before opening.

Détails sur CDK6

Antigène: CDK6 (Cyclin-Dependent Kinase 6 (CDK6))

Autre désignation: CDK6

Sujet: Cdk6 is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of Cdk6 first appears in mid-G1 phase, and is controlled by regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb.Synonyms: Cell division protein kinase 6, PLSTIRE, Serine/threonine-protein kinase PLSTIRE

ID gène: 1021

NCBI Accession: NP_001138778

UniProt: Q00534

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases