beta-2 Microglobulin anticorps (HRP)

N° du produit ABIN112400

Il existe 4+ publications pour ce produit. L’anticorps anti-beta-2 Microglobulin Monoclonal Souris est utilisé pour la détection de beta-2 Microglobulin dans des échantillons de Humain. Il a été validé pour WB, FACS, IP, IHC (p), RIA et EIA.

Aperçu rapide pour beta-2 Microglobulin anticorps (HRP) (ABIN112400)

Antigène: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp beta-2 Microglobulin est conjugé à/à la HRP

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA), Enzyme Immunoassay (EIA)

Clone: B2M-01

Détail du produit anti-beta-2 Microglobulin anticorps (HRP)

Specificité: The antibody reacts with beta-2-Microglobulin (B2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta-2-Microglobulin.

Réactivité croisée (Details): Species reactivity (tested):Human.

Immunogène: Purified Human beta-2-Microglobulin.

Isotype: IgG2a

Information d'application

Indications d'application: The antibody is designed for quantitative determination of soluble beta2-Microglobulin/MHC Class I complexes. This antibody can also be used for detection offree beta 2-Microglobulin by Western blotting. ELISA. Western blot (Both Reducing and non-Reducing conditions): Recommended Dilution: 2-4 μg/mL. Positive Control: U937 human histiocytic lymphoma cell line, RAJI human Burkitt lymphomacell line. Negative Control: EL4 mouse lymphoblastic lymphoma cell line. Sample Preparation: Resuspend approx.50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysatewith non-reducing/reducing Laemmli SDS-PAGE sample buffer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, 0.01 % (w/v) Thimerosal, 1 % (w/v) high-grade protease free BSA

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: This product is photosensitive and should be protected from light

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Références pour anticorps anti-beta-2 Microglobulin (HRP) (ABIN112400)

Poláková, Železníková, Russ: "HLA-G5 in the blood of leukemia patients and healthy individuals." dans: Leukemia research, Vol. 37, Issue 2, pp. 139-45, (2013) (PubMed).

Khurana, Traum, Aivado, Wells, Guerrero, Grall, Libermann, Schachter: "Urine proteomic profiling of pediatric nephrotic syndrome." dans: Pediatric nephrology (Berlin, Germany), Vol. 21, Issue 9, pp. 1257-65, (2006) (PubMed).

Lafon, Prehaud, Megret, Lafage, Mouillot, Roa, Moreau, Rouas-Freiss, Carosella: "Modulation of HLA-G expression in human neural cells after neurotropic viral infections." dans: Journal of virology, Vol. 79, Issue 24, pp. 15226-37, (2005) (PubMed).

Tökés, Hortoványi, Csordás, Kulka, Mózes, Hatalyák, Kádár: "Immunohistochemical localisation of tenascin in invasive ductal carcinoma of the breast." dans: Anticancer research, Vol. 19, Issue 1A, pp. 175-9, (1999) (PubMed).

Détails sur beta-2 Microglobulin

Antigène: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Autre désignation: beta-2-Microglobulin

Sujet: B2M non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.Synonyms: B2M, Beta 2 microglobulin, Beta-2 microglobulin

ID gène: 567

NCBI Accession: NP_004039

UniProt: P61769

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process