beta-2 Microglobulin anticorps

N° du produit ABIN112401

Cet anticorps anti-beta-2 Microglobulin Monoclonal Souris (Clone B2M-01) (ABIN112401) détecte spécifiquement beta-2 Microglobulin dans WB, FACS, IP, IHC (p), RIA et EIA. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour beta-2 Microglobulin anticorps (ABIN112401)

Antigène: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp beta-2 Microglobulin est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA), Enzyme Immunoassay (EIA)

Clone: B2M-01

Détail du produit anti-beta-2 Microglobulin anticorps

Specificité: The antibody reacts with beta-2-Microglobulin (B2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta-2-Microglobulin.

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein A

Immunogène: Purified Human beta-2-Microglobulin

Isotype: IgG2a

Information d'application

Indications d'application: Flow CytometryDilution: 1 mg/mLPositive control: PBL cell line,Negative control: DAUDI human lymphoma cell line, erythrocytes. ImmunoprecipitationB2M-01 coprecipitates beta2-microglobulin (12 kDa) and HLA antigens (45 kDa) from thelysates of human lymphoid cells. RIAThe dissociation constant of the antibody soluble beta2-Microglobulin is 1.5 x 10e-8 Mol/las determined by competitive RIA. ELISA. Immunohistochemistry (Paraffin)Dilution: 10 μg/mL. Positive tissue: kidney glomeruli. Western blotting (Reducing and Non-Reducing Conditions). Dilution: 2-4 μg/mL. Positive control: U937 human histiocytic ymphoma cell line. Negative control: EL4 mouse lymphoblastic lymphoma cell line. Sample Preparation (See Protocols).

Protocole: Sample Preparation (Western Blotting): Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mMTris/Cl, 100 mM NaCl pH 8. 2, 50 mM NaF including Protease inhibitor Cocktail). Incubate60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducingLaemmli SDS-PAGE sample buffer.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur beta-2 Microglobulin

Antigène: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Autre désignation: beta-2-Microglobulin

Sujet: Beta-2 Microglobulin non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.Synonyms: B2M, Beta 2 microglobulin, Beta-2 microglobulin

ID gène: 567

NCBI Accession: NP_004039

UniProt: P61769

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process